ABI 7300 Real-Time PCR

The Applied Biosystems 7300 Real-Time PCR System is optimized for use with the following standard dye sets: FAMTM/SYBR® Green I, VIC®/JOETM, NEDTM, TAMRATM, and ROXTM.

Applications include gene expression analysis, absolute quantitation, SNP genotyping, and +/– assays utilizing internal positive controls.


Advantages of real-time PCR include:

  1. • Ability to monitor the progress of the PCR reaction as it occurs in real time

  2. • Ability to precisely measure the amount of amlplification at each cycle, which allows highly accurate quantification of the amount of starting materials in samples

  3. • An increased dynamic range of detection

  4. • Amplification and detection occurs in a single tube, eliminating post-PCR manipulations